Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLCREVWIE-VNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEE-LLEVVKALKASKFLRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIKQLNLVKL-EPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV 1BD0 Chain:A ((4-375)) ----FHRDTWAEVDLDAIYDNVENLRRLLPDDTHIMAVVKANAYGHGDVQVARTALEAGASRLAVAFLDEALALREKGIEAPILVLGASRPADAALAAQQRIALTVFRSDWLEEASALYS-GP--FPIHFHLKMDTGMGRLGVKD-EEETKRIVALIERHPHFVLEGLYTHFATADEVNTDYFSYQYTRFLHMLEWL---PSRPPLVHCANSAASLRFPDRTFNMVRFGIAMYGLAPSPGIKPLL-PYPLKEAFSLHSRLVHVKKLQP-GEKVSYGATYTAQTEEWIGTIPIGYADGWLRRLQH-FHVLVDGQKAPIVGRICMDQCMIRLPG--PLPVGTKVTLIGRQGDEVISIDDVARHLETINYEVPCTISYRVPRIFFRHKRIM---------

General information: TITO was launched using: RESULT: Template: 1BD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2107 -64575 -30.65 -175.00 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -30.65 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_1BD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BD0-query.scw PDB file : Tito_Scwrl_1BD0.pdb: