Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFEADSLLFEADKRTKEYKELRSQMVKLKKAFKEVANLDDSEFSGKGADNIKAFYHGHVGVTDQWIDLIDMKIAFLSSMSATLEDAKMSDAYIEESFLEHELANAYAKSKSIMSEQKKAMKDILNNINDILPLEIFSTEDFKDKLSSADDKREKTIDKLNKLDEDLKTEYAETEPNEQFIQQDFKKLQESTGKGKNATPIHYNAKAYRESDIHKKKGDIEKHSEAYLSVKK-EEAKEREIKELKKKLNDGVSDPDEYLE---IAKKVGYENLEPTQVQLAVQIEQAKQLEGAGEITWDIVKGVGVGLYDVGKDTVTGIWDFITDPGETLSALGNAAMHPVKTYDAISAAIEESYQKDMVNGDAYSRSRWVTYAIGSVAVAVVGTKGAGAINKADAAGKVINKASQAGKKIKDVKIPDLLPYNPKYKLALADNVPYNVVDSQNLKNELLTNAKKI--PDGTRKPFTGQKKSPPWLNKEKYDAYEIEGKVKAKGKVKDVSRRVYTMKDIDINQKTEFGVTNLQLMKNGNAPYAKDGTQINLHHLIQEEPGPMLEIPNSLHTKYSDVIHQLKSDGESFRNDKVLKAQYESFRKRYWKWRAKQFENEN 1ZTM Chain:A ((4-466)) ITKLQHVGVLVNSPKGMKISQNFETRYLILS--------------------------------------------------------------------LIPKIEDSNSCGDQQIKQYKRLLDRLIIPLYDGLRLQKDVI---VSDIEKLKEAIRDTNKAVQSVQSSIGNLIVAIKSVQDYVNKEIVPSIARLGCEAAGLQLGIALTQHYSELTNIFGDNIGSLQEKGIKLQGIASLYRTNITEIFTTSTVDKYDIYDLLFTESIKVRVIDVDLNDYSITLQVRLPLLTRLLN---TQIYRVDSISYNIQ--NREWYIPLPSHIMTKGAFLGGADV------------------------------------KECIEAFSSYICPSDPGFVLNHEMESCLSGNISQCPRTVVKS-------DIVPRYAF---------------VNGGVVANCITTTCTCNGIGNRINQPPDQG-------------------------------VKIITHKECNTIGINGMLFNTNKEGTLAFYTPN-------------DITLNNSVALDPIDISIELNKAKSDLEESKEWIRRSNQKLDSI------------

General information: TITO was launched using: RESULT: Template: 1ZTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1415 36274 25.63 88.47 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 25.63 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.204

(partial model without unconserved sides chains): PDB file : Tito_1ZTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZTM-query.scw PDB file : Tito_Scwrl_1ZTM.pdb: