Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLHVQTASSSYPVFIGQGIRKKA-CELLTSLNRPLTRIMFVTDEEVDRLYGDEMLHLLQEKWPVKKVTVPSGEQAKSMDMYTKLQSEAIRFHMDRS-SCIIAFGGGVVGDLAGFVAATFMRGIDFIQMPTTLLAH-DSAVGGKVAVNHPLGKNLIGAFYQPKAVLYDTDFLRSLPEKELRSGMAEVIKHAFIYDRAFLEELLN-IHSLRDI-TNDQLNDMIFKGISIKASVVQQDEKEEGIRAYLNFGHTLGHAVEAEYG-YGQITHGDAVALGMQFALYISEKTVGCE-MDRKRLVSWLKSLGYPSQIRKETETSVLLNRMMNDKKTRGGKIQFIVLNELGKVADHTFSRNELESWLNKWRLEETS 5HVN Chain:A ((13-360)) -------TFSPSYNIIVDSVL--DFSHILE---YVTNKQVLVVTNTTVAKLYLTKFLAALVDDLDVRTCILEDGEQYKSQQSLDKILSTLLENHFTRNSTVLVALGGGVIGDITGFAAAIYQRGIDFIQIPTTLLSQVDSSVGGKTAINHQLGKNMIGAFYQPKVVYTSIEFYKTLPQREYIAGMAEVVKYAFIS-KDFYLWLDSNRDKILAKDSVTLI-EMVKRSCQIKAQVVAMD---TGARAILNFGHTFGHAIEKCQNYRG-LKHGEAVGVGMAQAIDFSHYLGLISQQQAKDFNDFIVSFGISIDFPNDICQKEFLEAMLLD----NKELKFILIENIGSLSLQKQSKNELEQFLDI-------

General information: TITO was launched using: RESULT: Template: 5HVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1785 -49286 -27.61 -148.00 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -27.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_5HVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HVN-query.scw PDB file : Tito_Scwrl_5HVN.pdb: