Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRGQPVYLSYAGTAEDAHLILSRLSNYSMYTLEEELKKGYVTIRGGHRVGLAGRVITENGGVKGLRDIASFNIRIARQKLGIAEPLLPYLYQN--SWLNTLIIGPPQTGKTTLLRDLARLSSTGKKNM--LPV------------KTGIVDERSEIAGCL--RGI----PQ---HQ----------------F-----------GQRIDVLDACPKAEGLMMMIRS--MSPEVMIVDEIGRMEDTDA---LLEAL---HAGVSVIVSAHGWSISDLMKRPSLKRLWEERAFDRYLELSRAKG--PGTVSQIYDKDGNVLSRTTGVKTC 3ZDQ Chain:A ((359-571)) ------------------------------------------------------------------------------------------------------------------------------IFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQLNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEKGVLLSGGQKQRI--AIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTI--------------KNANMVAVLDQGKITEYGKHEELLSK--------------

General information: TITO was launched using: RESULT: Template: 3ZDQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 596 -9625 -16.15 -63.74 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -16.15 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_3ZDQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZDQ-query.scw PDB file : Tito_Scwrl_3ZDQ.pdb: