Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKSILKIAAAACDDKRAEDILALDMEGISLVADYFLICHGNSDKQVQAIAREIKDQADENGIQVKKMEGFDEARWVLVDLGDVVVHVFHKDERSYYNLEKLWGDAPLADLDFGMNQ 2LN3 Chain:A ((1-83)) MGLTRTITS-------QNKEELLEIALKFISQGLDLEVEFDSTDDKEIEEFERDMEDLAKKTGVQIQKQWQGNKLRIRLK--GSLEHHHHHH--------------------------

General information: TITO was launched using: RESULT: Template: 2LN3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 313 -25252 -80.68 -304.24 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -80.68 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2LN3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LN3-query.scw PDB file : Tito_Scwrl_2LN3.pdb: