Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence KRLVVQRVTEASVTVDEEVVGQ-------IGQGLMVLVGITHDDTEDDAAYLADKVVNLRIFDDSEGKMNLSLVDIGGEILSVSQFTLYGDTKKGRRPNYMNAAKPDKALGLYEKWNDLLREK--GIKVETGTFGAMMDVQLTNSGPVTLIMDSK 3KOC Chain:D ((2-157)) -RVVIQRVKGAILSVR------ELEIISEIKNGLICFLGIHKNDTWEDALYIIRKCLNLRLWNNDNKTWDKNVKDLNYELLIVSQFTLFGNTKKGNKPDFHLAKEPNEALIFYNKIIDEFKKQYNDDKIKIGKFGNYMNIDVTNDGPVTIYIDT-

General information: TITO was launched using: RESULT: Template: 3KOC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 600 12656 21.09 91.71 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.83 3D Compatibility (PKB) : 21.09 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_3KOC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KOC-query.scw PDB file : Tito_Scwrl_3KOC.pdb: