Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKNVLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIFSKKVTAEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPL------QDRMCFAVLQTVIETAPK-------LLEDLENYELRETILYAG-TIALNGTLQMG-YFGDWASHTMEHAVSA---VYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKE---MEHGGFGNFQKLNKDDVLAILRASL 4MCA Chain:A ((2-355)) --LRIIQSPGKYIQGANALAAVGQYAKSLADHYLVIADDFVMKLA--GDTLMGSLQQHGVKHH-AALFNGECCHKEIDRLGRELKAHGCRGVIGVGGGKTLDTAKAIAHY------------------QQLPVVLIPTIASTDAPTSALSVIYTEQG-EFAEYLIYPRNPDMVVMDVAIIAKAPVRLLVAGMGDALSTYFEAQACFDAQ-ATSMAGGKSTLAALSLARLCYDTLLAEGVKAKLAVEAGVVTEAVERIIEANTYLSGIGFESSGLA----AAHAIHNGFTVLEECHHLYHGEKVAFGTLAQ--------------LVL--QNS----------PMAQIETVLAFCHRIGLPITLAEMGVSGDAVEKIMAVAQASCAAGE-TIHNMPFKVTPAGVQAAILTA-

General information: TITO was launched using: RESULT: Template: 4MCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1981 -160129 -80.83 -480.87 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -80.83 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_4MCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MCA-query.scw PDB file : Tito_Scwrl_4MCA.pdb: