Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYIITGASKGLGQAIALQALEKGHEVHALSRTKTDV--SHK---KLTQHQIDLINLEEAEQQFETLLSSIDSDRYSGITLINNAGMVTPIKRAGEASLDELQRHYQLNLTAPVLLSQLFTKRFASYSGKKTVVNITSGAAKNPYKGWSAYCSSKAGLDMFTRTFGFEQEDEELPVNMISFSPGVMDTEMQAVIRSSSKKDFHHIERFRKLNETGSLRSPDFIAGTLLSLLEKG--TENGRIYDIKEFL 3D3W Chain:A ((7-238)) GRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGS----V----GPVDLLVNNAAVALL-QPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELG--PHKIRVNAVNPTVVMTSMGQATWSD----PHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMTTGSTLPVE---

General information: TITO was launched using: RESULT: Template: 3D3W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1181 -14134 -11.97 -62.82 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -11.97 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_3D3W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D3W-query.scw PDB file : Tito_Scwrl_3D3W.pdb: