Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTICVFAGSNPGGNEAYKRKAAELGVYMAEQGIGLVYGGSRV-GLMGTIADAIMENGGTAIGVMPSGLFSGEVVHQNLTELIEVNGMHERKAKMSELADGFISMPGGFGTYEELFEVLCWAQIGIHQKPIGLYNVNGYFEPMMKMVKYSIQEGFSNESHLKLIHSSSRPDELIEQMQNYSYPILEKKWTEI 2A33 Chain:A ((13-190)) FRRICVFCGSSQGKKSSYQDAAVDLGNELVSRNIDLVYGGG-SIGLMGLVSQAVHDGGRHVIGIIPKTLM--------VGEVRAVADMHQRKAEMAKHSDAFIALPGGYGTLEELLEVITWAQLGIHDKPVGLLNVDGYYNSLLSFIDKAVEEGFISPTAREIIVSAPTAKELVKKLEE-------------

General information: TITO was launched using: RESULT: Template: 2A33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 865 -43799 -50.63 -259.17 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -50.63 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_2A33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A33-query.scw PDB file : Tito_Scwrl_2A33.pdb: