Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIKEHLKQLKPYQPGKPIEAVKSEYGLDKVVKLASNENPYGCSEAAKEALHHEIQQLALYPDGYSAALRTRLSKHLNVSETSLIFGNGSDEIIQIICRAFLNNK-TNTITAAPTFPQYKHNAVIEGAEVREIALRPDGSHDLDAMLEAIDEQTQVVWICSPNNPTGTYTSEGELLAFLERVPSRVLVVLDEAYYEYVTAEDYPETVPLLSKYSNLMILRTFSKAYGLAALRVGYGIADENLIRQIEPAREPFNTSRLGQAAAIAALDDQ--AFIASCVEQNNAGLQQYYDFAKTH-GL-KCYPSQTNFVLIDFK-RPADELFQALLEKGYIVRSGNAL-GFPTSLRITIGTKEQNEEILAILAEIL 1FG7 Chain:A ((10-354)) --ARENVRNLTPYQSARRL-------GGNGDVWLNANEYPTAVEFQL----T--QQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDAILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKL-DGVKVVYVCSPNNPTGQLINPQDFRTLLELTRGKAIVVADEAYIEFCPQA---SLAGWLAEYPHLAILRTLSKAFALAGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAEREYLIAALKEIPCVEQVFDSETNYILARFKAS--SAVFKSLWDQGIILRDQNKQPSLSGCLRITVGTREESQRVIDALRAE-

General information: TITO was launched using: RESULT: Template: 1FG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1868 -64256 -34.40 -190.11 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -34.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_1FG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FG7-query.scw PDB file : Tito_Scwrl_1FG7.pdb: