Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAVIEVQELKEEVAG-TLAVGSTIYCAALMLEKVTQ-IKERYPHLTFNIWENEPATLLELLESRQIDAAVTTTLIKSDT---VQFKQLDDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNLEPRIVCECHDSATLLSLVSSGFGASILPVTMIPVHMRN-HVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF----- 2ESN Chain:A ((3-303)) PLLRRLDLNLLLVFDALYRHRNVGTAASELAISASAFSHALGRLRQGLDDELFLRQGN-RMQPTQRAEHLAAAVAAALRALGEGLEEWRPFVPGQSQRTFVFAATDYTAFALLPPLMNRLQHSAPGVRLRLVNAERKLSVEALASGRIDFALGYDEEHERLPEGIQAHDWFADRYVVVARRDHP-RLAGAPTLEGYLAERHAVVTPWNED--SGVIDRLLARSGLRREVAVQLPTVLAALFLAGSTDFLLTAPRHAARALAEAAGLALYPAPFDIPPYVLRLYSHVQG---RDAHAWMIGQLKGLD

General information: TITO was launched using: RESULT: Template: 2ESN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1267 -204697 -161.56 -723.31 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -161.56 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_2ESN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ESN-query.scw PDB file : Tito_Scwrl_2ESN.pdb: