Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKASVIIPAYNSKERLYNSLLSLNQQECDEEFEVIVADNGSEDG------TLSMLESFQA--DFPLIFTRI----------------------KENRGIAYGRNQALRNA---RGDILIFHDSDMLAAKDLVA----KHI-KAHENE-ENLVVCGLFWKRIYSFYYERFEEEHKEQLAKLTGEMPKKDKQKLLEEADIKNGSFLDKSFDLDTDFIDVLKKILDEYGDDLKGYHMPWRFFITNNSSVKRKHVVDLGLFDEGIVRYGFEDYDLGIRLHQAGLTFRLRRDIVSVHQEHPSNCKSVDDIRANIAYMCDKYNNIRSLDVHLAFNGPFPPDMTNRIMADIQKLLESQKYDMLLNLFLELLHVVKERNIDPDWRKKSPRVTAKSFDLQTVRKLLPKAKKKLGVNDFANALYALVNDLLHVDLRSLDVV 2ZU9 Chain:A ((51-220)) GKMAVIVPMKNEKL---HLVDGVLKAIPH-KCPIIIVSNSKREGPNRYKLEVDLIRHFYNLTHSKIIMIHQKDPGLAKAFKEVGYTDILDENGMIRSGKGEGMLVGLLLAKAIGAEYVGFVDAD-NYIPGAVNEYVKDYAAGFLMSESEYTMVRLHWR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 467 -62434 -133.69 -547.66 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -133.69 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_2ZU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZU9-query.scw PDB file : Tito_Scwrl_2ZU9.pdb: