Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANVLMIGFPGEGHINPSIGVMKELKSRGENITYYAVKEYKEKITALDIEFREYHDFRGDY--FGKNA---TGDEE------R-DFTEMLCAFLKACKDIATHIYEEVKHESYDYVIYDHHLLAGKVIANMLKLPRFSLCTTFAMNEEFAKEMMGAYMKGSLEDSPHYESYQQLAETLNADFQAEIKKPFDVFLADGDLTIVFTSRGFQP--LAEQFG-ERYVF-VGPSITERAGNNDFPFDQIDNENVLFISMGTIFNN--QKQFFNQCLEVCKDFDGKVVLSIGKHIKTSELNDIPENFIVRPYVPQLEILKRASLFVTHGGMNSTSEGLYFETPLVVIPMGGDQFVVADQ--VEKVGAGKVIKKEELSESLLKETIQEVMNNRSYAEKAKEIGQSLKAAGGSKKAADSILEAVKQKTQSANA 3OTI Chain:A ((20-396)) HMRVLFVSSPGIGHLFPLIQLAWGFRTAGHDVLIAVAEH-ADRAAAAGLEVVDVAPDYSAVKVFEQVAKDNPRFAETVATRPAIDLEEWGVQIAAVNRPLVDGTMALVDDYRPDLVVYEQGATVGLLAADRAGVPAVQRNQSAWRT-R-----GM--HRS-------IAS--FLTDLMD-KHQVSL--------PEPVATIESFPPSLLLEAEPEGWFMRWVPYGGGAVLG-----DRLP--PVPARPEVAITMGTIELQAFGIGAVEPIIAAAGEVDADFVLALGDLDIS-PLGTLPRNVRAVGWTPLHTLLRTCTAVVHHGGGGTVMTAIDAGIPQLLAPDPRDQFQHTAREAVSRRGIGLVSTSDKVD----ADLLRRLIGDESLRTAAREVREEMVALPTPAETVRRIVERI---------

General information: TITO was launched using: RESULT: Template: 3OTI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2095 -23474 -11.20 -65.75 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -11.20 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.518

(partial model without unconserved sides chains): PDB file : Tito_3OTI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OTI-query.scw PDB file : Tito_Scwrl_3OTI.pdb: