Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNEKRLLEEFLELVQIDSETK----------HEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGT--KQTDTIYFTSHMDTVVPGNGV--KPVV-----E-------------------NGYVKTD------GTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGYALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEP-EKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCDEVHILAEARSLVPEKMEAQVQKMKAAFEEAAADM--GGRAEVEIEVMYPGF-KYQDGDQ-VVEIAKKAAAKIGRPSELQTSGGGSDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK 1FNO Chain:A ((2-405)) ----DKLLERFLHYVSLDTQSKSGVRQVPSTEGQWKLLRLLKQQLEEMGLVNITLS-------EKGTLMATLPANVEGDIPAIGFISHVDTSPDFSGKNVNPQIVENYRGGDIALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGVAEIMTALAVLKGNPIPHGDIKVAFTPDEEVGK-GAKHFDVEAFGAQWAYTVDGGG-VGELEFENFNAASVNIKIVGNNVHPG-TAKGVMVNALSLAARIHAEVPADEA-PETTEGYEGFYHLASMKGTVDRAEMHYIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCHITPEMKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKAVQVIVRIAELTA-

General information: TITO was launched using: RESULT: Template: 1FNO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1671 49807 29.81 140.30 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 29.81 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_1FNO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FNO-query.scw PDB file : Tito_Scwrl_1FNO.pdb: