Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIEHYVALVKTGCAEYGQMNEMTLTEIADCLFCTERNAKLILHKLENSNWIIRESGAGRGRKSKIAFLRRPEELLLQTAKEYTMSGKLKKAKELLQQYQSAFPGLQNEYNMWLSEVFGFVTETGENGAKDVLRLFITPESVSSLDPCQIFLRSEGHFVKQIFDTLFTFDSDMQEPKPHLVHGWEEVG-KKQWRFFLRKGVLFHNGQPLTSRDVAFTFQRFLELA-DNPYKWL---LHGVKQVLEKGPYCVELILDKPNALLPYALCDERLSILPAEQG------------GGKNGTGPFQMNQQH-SGMLVLEANERYFKGRPYLDRVEFVFSEQAGE-MN---GFTIQ--------E---KQTCPEQQTVFDERHVQYLSLNLKKKGPLQHRSFRKALRLLISSERLVREAGGH--RRIPVTSFLHPSP---------FEWEGVSPSELLKKSGYE-GETIVLYTFS-ETDHREDAEWIQNICAQHGIRLTLQFCDAADLRRP---EIVQMADIIHDSATFYQDSEFGFLHLLLSENSFLYQHLSEKLTQICSGMTERMFSMPDRCSRINILRDIDRQMIQELNAIPLYQNVLQVTSSKNVKGLVLDE----EGWIDLYSVWLSK 5ISU Chain:A ((15-502)) --------------------------------------------------------------------------------------------------------------------------------KDGGSLIIGV-TGDPEVINPNYASDRVTLTIQQAVYAPLFWEVD--G--KPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVFTVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLPTVAPAFENTIKT--FFPIPKHIFEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQNAANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQ-PALKSKELRQALSYALDREEIIDAAYGSDEYAKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDTSQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDPNALSNITLDRKNADYSIALNGYIMGNDPD-AYKSLYLSDAPYNYSNYH---NKDLDALWEKGAVTADDKERQEIYEKIQNTIADDAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE

General information: TITO was launched using: RESULT: Template: 5ISU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2243 -159383 -71.06 -366.40 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -71.06 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_5ISU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ISU-query.scw PDB file : Tito_Scwrl_5ISU.pdb: