Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGMEWFPLLGLANRARKVVSGEDLVIKEI--RNARAKLVLLTEDASSN-------TAKKVTDKCNYYKVPYKKVESRAVLGRSIGKE---------------ARVVVAVTDQGFANKLISLLD 2KG4 Chain:A ((19-134)) -VGDALEEVLSKALSQRTITVGVYEAAKLLNVDPDNVVLCLLAADEDDDRDVALQIHFTLIQAFCCENDINILRVSNPGRLAELLLLETDAGPAASEGAEQPPDLHCVLVTNPHSSQ-------

General information: TITO was launched using: RESULT: Template: 2KG4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 349 -58597 -167.90 -636.92 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -167.90 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_2KG4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KG4-query.scw PDB file : Tito_Scwrl_2KG4.pdb: