Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIKIKYLDETQTRINKMEQGDWIDLRAAEDVAIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTKIKKGDRICQFRIMKKMPAVDLIEVDRLGNGDRGGHGSTGTK 4AOZ Chain:C ((3-144)) MQIKIKYLDETQTR---IEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK

General information: TITO was launched using: RESULT: Template: 4AOZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 531 -64556 -121.57 -464.43 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.98 3D Compatibility (PKB) : -121.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4AOZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AOZ-query.scw PDB file : Tito_Scwrl_4AOZ.pdb: