Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRGQPVYLSYAGTAEDAHLILSRLSNYSMYTLEEELKKGYVTIRGGHRVGLAGRVITENGGVKGLRDIASFNIRIARQKLGIAEPLLPYLYQN--SWLNTLIIGPPQTGKTTLLRDLARLSSTGKKNM--LPV------------KTGIVDERSEIAGCL--RGIPQ----H---QF-------------G-----------QRIDVLDACPKAEGLMMMIRS--MSPEVMIVDEIGRMEDTDA---LLEALH-----AGVSVIVSAHGWSISDLMKRPSLKRLWEERAFDRYLELSRAKG--PGTVSQIYDKDGNVLSRTTGVKTC 4MYC Chain:A ((450-662)) -----------------------------------------------------------------------------------------------------------------------------KILKNASFTIPAGWKTAIVGSSGSGKSTILKLVFRFYDPESGRILINGRDIKEYDIDALRKVIGVVPQDTPLFNDTIWENVKFGRIDATDEEVITVVEKAQLAPLIKKLPQGFDTIVGERGLMISGGEKQRL--AIARVLLKNARIMFFDEATSALDTHTEQALLRTIRDNFTSGSRTSVYIAHRLRTI--------------ADADKIIVLDNGRVREEGKHLELLAM--------------

General information: TITO was launched using: RESULT: Template: 4MYC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 568 7266 12.79 47.80 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 12.79 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_4MYC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MYC-query.scw PDB file : Tito_Scwrl_4MYC.pdb: