Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMRHKPWADDFLAENADIAISNPADYKGKWNTVFGNDNPIHIEVGTGKGQFISGMAKQNPDINYIGIELFKSVIVTAVQKVKDSEA-------QNVKLLNIDA-DTLTDVFEPGEVKRVYLNFSDPWPKKRHEKRRLTYSHFLKKYEEVMGKGGSIHFKTDNRGLFEYSLKSFSEYGLLLTYVSLDLHNSNLEGNIM--TEYEEKFSALGQPIYRAEVEWRT----- 2VDV Chain:E ((14-240)) NPFSDHQLEYPVSPQDMDWSKLYPYYKNQ------MTKKVTIADIGCGFGGLMIDLSPAFPEDLILGMEIRVQVTNYVEDRIIALRNNTSKHGFQNINVLRGNAMKFLPNFFEKGQLSKMFFCFPDPH------KARIITNTLLSEYAYVLKEGGVVYTITDVKDLHEWMVKHLEEHPLFERLSKEWEENDECVKIMRNATEEGKKVERKKGDKFVACFTRLPTPAIL

General information: TITO was launched using: RESULT: Template: 2VDV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 900 165 0.18 0.82 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain E : 0.66 3D Compatibility (PKB) : 0.18 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_2VDV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VDV-query.scw PDB file : Tito_Scwrl_2VDV.pdb: