Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHF-PGNDYENMMRVRMAANDLPDLFDTHG--WGKIRYGEYTADLRDMKW------TQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIA---PPETMDDFIKALRTIKEKSKG-SIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQW-S-KFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQINPNVKVGIIPTPAIHPGDDPIWIGGERYTLAAWKDSPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKA-DIFYA-NDY---EYYQDVKVEPYFD-RLYLPNGMWD-VLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE 4RJZ Chain:A ((26-405)) ------------------------------VTLTLWSLDR---DIQPAPNLIKEFNALNNGIKIEYRQLQFDDVVSESMRAYSTGNAPDIIAIDNPNHAMFASRGAFLDVTDMIAKSDVIKTENYFPGPLKS-VTWDGKYFGVPKATNTIALYYNKDLFKAAGLDAAKPPQTWDELVDAARKLT-N-PAKNVYGISFSAKANEEGTFQFLPWAQM-----AGATY--------KNINTDGAVKALETWKTLLDEKLASPDTLTRSQWDSTATFNAGNAAMAISGPWEIDRMLKD-AKFDWGVTLLPVPTPDAPRSSA-MGDYNWAIFSKTKHPAEAFKAIEFFASKDKDM-F-KNFGQLPARSDIPVPPTGNALKDEALKTFVEQLKYAQPRGPSPEWP--KISKAIQDAIQGALSGQMTPKAALDQAAEKIKLV-------------

General information: TITO was launched using: RESULT: Template: 4RJZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2038 40396 19.82 112.84 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 19.82 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4RJZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RJZ-query.scw PDB file : Tito_Scwrl_4RJZ.pdb: