Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKRKIGLAMSLVIAAGTILGACGNSEKSSGSGEGKNKFSVAMVTDVGGVDDKSFNQSAWEGIQAFGKENGLKKGKNG-YDYLQS-KSDADYTTNLNKLARENFDLIYGVGYLME-DSISEIADQRKNTNFAIIDAVVDKDNVASITFKEQEGSFLVGVAAALSSKSGKIGFVGGME-SELIKKFEVGFRAGVQAVNPKAVVEVKYAGGFDKADVGKATAESMYK--SGVDVIYHSAGATGTGVFTEAKNLKKEDPKRDVWVIGVDKDQYAEGQVEGTDDNVTLTSMVKKVDTVVEDVTKKASDGKFPGGETLTYGLDQDGVGISPSKQNLSDDVIKAVDKWKKKIIDGLEIPATEKELKTFKAE 3DBI Chain:A ((60-310)) ------------------------------------STQTLGLVV-TNTLYHGIYFSELLFHAARMAEEK------GRQLLLADGKHSAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDA-HSQPIMVLNRRLRKNSSHSVWCDHKQTSFNAVAELINA-GHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIANGKW-TPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVP-EQVSVIGFDDIAIAP------YTVPALSSVKIPVTEMIQEIIGRLIF-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3DBI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1280 1722 1.35 7.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 1.35 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_3DBI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DBI-query.scw PDB file : Tito_Scwrl_3DBI.pdb: