Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGVIDYGMGNLYSVSKALERVGVPYFVSEKPEELKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICLGMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTE-QGYAYFVHSYYIDGMEE-NALLASADYGVRVPAVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK 2NV0 Chain:A ((3-194)) TIGVLGLQG-AVREHIHAIEACGAAGLVVKRPEQLNEVDGLILPGGESTTMR-RLIDTYQFMEPLREFAAQGKPMFGTCAGLIILAKEIA-----PHLGLLNVVVERNSF---------GRQVDSFE-----ADLTIKGLDEPFTGVFIRAPHILEAGENVEVLSEHN-G--RIVAAKQGQFLGCSFHPELTEDH--RVTQLFVEMVEEYKQKA

General information: TITO was launched using: RESULT: Template: 2NV0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1086 -88314 -81.32 -474.80 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -81.32 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_2NV0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NV0-query.scw PDB file : Tito_Scwrl_2NV0.pdb: