Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNQVMLVGRLTKDPDLRYTSAGAAVAHVTLAVNRSFKNA-SG--EIEADYVNCTLWRK-TAENTALYCQKGSLVGVSGRIQTRSYENEEGVNVYVTEVLAD----TVRFMDPKPREKAAD 3ULP Chain:A ((81-194)) SLNKIMLIGRVGCEPDIKILNGGDKVATFSLATNEFWRDRNTNELKSKTDWHRIVVYDQNIVDLIDKYLRKGRRVYVQGSLHTRKWHT--SQPKQITEIILSYNKGDLIFLD---------

General information: TITO was launched using: RESULT: Template: 3ULP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 370 -16892 -45.65 -165.61 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -45.65 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_3ULP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ULP-query.scw PDB file : Tito_Scwrl_3ULP.pdb: