Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITESRQMLDKRNRPLRDLRISVTDRCNFRCTYCMPAELFGPDYPFLKKEELLSFEELERLATLFVTRFGVEKIRLTGGEPLMRKD---MPELIKKLARIPGIRDIAMTTNGSLLP-VYAKRLKEAGLKRVTISLDSLEDERFKKING--RGVSVSKVLEGIEAAKQAGLG-VKINMVVQKGVNEKDILPMARYFKEKGHILRFIEFMDVGNTNQWEKKDVMTKAEIIDLINKHMPVEPIAPNYIGEVASRFRYLDGSGEIGVISSVSDAFCGSCNRARLSARGELFTCLFASSGFDLRAPVRQELSDDELSEMIGTVWKNRIDQYSVDRTLSKASGKKKVEMSYIGG 4RTB Chain:A ((98-293)) ----------------LFAPLYLSNICINNCSYCSFRRENKE-----LSRVRLSLEEAVDEAKAIR-EMGHTRILLVMGEEPEDKTLSYLEEIIPAIYSEVDI--RRINVNIAPLTLKGYERLKKLKIGTYQLFQESYNPEVYREVHLDGPKTNFLWRLNAVERAIEAGIDDIGIGALFGLGDPLFELLGVIAHADY-------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RTB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 766 -19565 -25.54 -117.86 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -25.54 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4RTB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RTB-query.scw PDB file : Tito_Scwrl_4RTB.pdb: