Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLAAGIIGLLTVSIASPSFAAEKQADTNVAVLFDGSGSMVQKTGGERKIDIAKKSVKSFAELL-PKDTNLMLRVFGHAGNNKLSGKALSCSTTETIYGLHPYEGSLFDNSLSELK---PTGWTPIAKALADTRKEFEAFD---ADGKNVVYLITDGEETCGG--DPAAEIEKLRASNVDTIVNIIGFNFDVKGNEEMKQAAVAGGGEYISANSADEFEQAWEKEAQKFTE 3N2N Chain:F ((5-184)) --------------------------YGGFDLYFILDKSGSVLHH------WNEIYYFVEQLAHKFISPQLRMSFIVFSTRG--------------TTLMKLT-EDREQIRQGLEELQKVLPGGDTYMHEGFERASEQIYYENRQGYRTASVIIALTDGELHEDLFFYSEREANRSRDLG--AIVYAVGVKDF--NETQLARIADSKDHVFPVNDGFQALQGIIHSILKKS--

General information: TITO was launched using: RESULT: Template: 3N2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 897 26432 29.47 154.57 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain F : 0.66 3D Compatibility (PKB) : 29.47 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_3N2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N2N-query.scw PDB file : Tito_Scwrl_3N2N.pdb: