Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTTTAKQ--------------ASTKFVVLGLLLGILMSAMDNTIVATAMGNIVADLGSFDKF-------AWVTASYMVAVMAGMPIYGKLSDMYGRKRFFLFGLIFFLIGSALCG----IAQTMNQLIIFRAIQGIG----GGALLPIAFTIIFDL---FPPEKRGKMSGMFGAVFG---------LSSVLGPLL---------GAIITDSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGAITLVVSIVCLMFAL-----ELGGKTYDWNSIQIIG---LFIVFAVFFIAFFIVE------RKAEEPIISFWMFKNRLFATAQILAFLYGGTFIIL----------AVFIPIFV--------QAVYGSSATSAGFILTPMMIGSVIGSMIGGIFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTV---------FMMISGFGVGFNFSLLPAASMNDLEPRFRGTANSTNSFLRSFGMTLGVTIFGTVQTNV---FTNKLNDAFSGMKGSAGSGAAQNIGDPQEIFQAGTRSQIP----DAILNRIIDAMSSSITYVFLLALIPIVLAAVTILFMGKARVKTTAEMTKKAN------------------------------------------------------------------- 5FRP Chain:A ((3-691)) KGAVTKLKFNSPIISTSDQLISTNELLDRLKALHEELASLDQDNTDLTGLDKYRDALVSRKLLKHKDVGIRAFTACCLSDILRLYAPDAPYTDAQLTDIFKLVLSQFEQLGDQENGYHIQQTYLITKLLEYRSIVLLADLPSSNNLLIELFHIFYDPNKSFPARLFNVIGGILGEVISEFDSVPLEVLRLIFNKFLTYNPNEIPEGLNVTSDCGYEVSLILCDTYSNRMSRHLTKYYSEIIHEATNDDNNSRLLTVVVKLHKLVLRLWETVPELINAVIGFIYHELSSENELFRKEATKLIGQILTSYSDLNFVSTHSDTFKAWISKIADISPDVRVEWTESIPQIIATREDISKELNQALAKTFIDSDPRVRRTSVMIFNKVPVTEIWKNITNKAIYTSLLHLAREKHKEVRELCINTMAKFYSNSLNEIERTYQNKEIWEIIDTIPSTLYNLYYINDLNINEQVDSVIFEYLLPFEPDNDKRVHRLLTVLSHFDKKAFTSFFAFNARQIKISFAISKYIDFSKFLNNQESMSSSQGPIVMNKYNQTLQWLASGLSDSTKAIDALETIKQFNDERIFYLLNACVTNDIPFLTFKNCYNELVSKLQTPSIMPRDIAKVIQILLFRASPIIYNVSNISVLLNLSNNSDAKQLDLKRRILDDISKVNPTLFKDQIRTLK

General information: TITO was launched using: RESULT: Template: 5FRP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1807 -341384 -188.92 -666.77 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -188.92 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_5FRP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FRP-query.scw PDB file : Tito_Scwrl_5FRP.pdb: