Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNETPFSK--NAEMYRDEKVFAEGEDLGLMIKTAEC----RAEHRVLDIGAGAGHTALAFSPY-------VQECIGVDATKEMVEVASSFAQEKGVENVRFQQGTAESLPFPDDSFDIITCRYAAHHFSDVRKAVREVARVLKQDGRFLLVDHYAPED-PVLDEFVNHLNR---------LRDPSHVRESSLSEWQAMFSANQLAYQDIQKWNL--PIQYDSWIKRGGTPADRE--KQI-ITHLNHASD----EARDTFCI-TLNQNGQPISFCLKAILIQGIKR 5EGP Chain:A ((36-314)) ----NMFQTKSFVDRYKYTEK-LTGLYAQTLVDYSGVANTSQKPLVVLDNACGIGAVSSVLNHTLQDEAKKTWKLTCGDLSEGMLETTKRRLQDEGWVNAETKIVNALDTGLPDGHYTHVFVAFGFQSFPDANAALKECFRILASGGILASSTWQNFNWIPIMKAAIETIPGNLPFPTQKEFITLHNAGWDSESYIQSELEKLGFRDVKVIPVPKETSIPIDEFFEVCMMIIPYLLPKFWTEEQRESHEKDVPMVLRQYLQDTYGA-N-GQVPLEAVALITTGLKP

General information: TITO was launched using: RESULT: Template: 5EGP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1312 -133818 -102.00 -543.98 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -102.00 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.350

(partial model without unconserved sides chains): PDB file : Tito_5EGP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGP-query.scw PDB file : Tito_Scwrl_5EGP.pdb: