Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------MKRITINIITMFIAAAVISLTGTAEAAEKQQQSPANVTLTDQQKKEIEQLEAEILKKRKDVISKYVQYGILPKER---GEHIKN-HLDKHFEMMKQNGFVPKHHPHPHKFEKRH-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 4HU8 Chain:A ((3-452)) NEITLTIGQQKDLASMVPAKFAGQELSWTSSDPETASVTDKGIVTALKFSSGGANLFLKAPATGEAIITVTA------GKQSHSVKVITTVKGKEDIEKL-----PPLKDHFKDYFLIGNIFNNRDVSGSMMDNDWLAHHYA-----ILTPENHMKPSNLTNNRNETTGEITYTFSTADRMVNAAIAEGLKIHGHTLLWHQQIPPWQRSMESAAKDAALSVMKKYITEVMTHYKGKIYSWDVLNEIFPDGRGDNWTTAMRPENPWFKSIGSDFVYEAYLAARQADPNAILYYNDYNMDQAGKAALIAAMVRDVNAKYKQAYPRETRLLIEGIGMQSHHNMDVPASNIRNTINRYRELGVKISVSELDILCMGWSAFRGSTGQGADKDDMTIATNRNILDQAYKFNEYMKLYLENSDIIERVSMWGVSDRYSWRSGGLPLLFDADNKAKPAYYSFVRAREDYEAAKA

General information: TITO was launched using: RESULT: Template: 4HU8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 196 -429 -2.19 -4.56 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -2.19 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_4HU8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HU8-query.scw PDB file : Tito_Scwrl_4HU8.pdb: