Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------MLCVMM-------LLFSAIASFPVS-AQAKDQDAGILIIYSTLDGKESSQVKMLDLLAGHFTS----HVTVKKDSDVEAS-----------------DFKGKDHVIYYGQTKRKLSQKLLSLISGVKK---------------PVVAIGYNAGQISQFSGLSLARKENVFQV-HSRSEKADVSLESG--------LNVLSVSGLKGTALYTFKADEGTTHSF------IWKT-----KKGNVYIGLTNLLNDNLIVAKQLREAFGEKAGTTLLY-----------LRLEDISPMSDEKLLLQAGTY--LHKRHIPFILAVIPVYLNPETGDKVYLSNQPKMVKVLKKLQSMGGSIIVHGYT----HAYRYSE-------TGEGFEFWDAKADQPITSGNAEDPPSILEKEQDFPNEQAYHSYLEPFR-------EKEETYTKQKLTRAIEDLTSS-GLYPLAFEAPHYTMSE-YGYQIASQYFTSIFGQVQLSSTTWKTSGAPPFVTAPSMLHGMTLYPETIGF--VDTSKQNPLGEMEEHISQMIDFEGGVAGGFYHPYLGMKYLPE----LVDQMERIPDSEWLDLKKTKQTVKTDKVEIHTSGDGTIQVKNGVSPI-YEFFDHHRQTPLEK--ALWILSAVVLLFVIMF---VSYTFYLRATLKKRIFKERRSLG-------------------- 5F7S Chain:B ((1-730)) MITHRPRGIEHPYARSLDQLYPAIPIAGQSLTIGATTSGPCSRMRCFVLWPEHEQVFDMSPVNGWQTSIPHLPDQDATYYFEALTLDGRTETS-ESFPLTPSHWSAEPVGHIDIDGDRFIPDSPLWLVSSAGTHRVKFALRIEGDEHVVGFGERYDQLDQRGLRLDSVVFEQYKAQGKHHRTYLPMPFAQVVNEAGRAWGFHVETTRR--TWYDVAATVSDRILIEVDLGFEAEKTPVVRVNTWSGSPTDVLNGFLDVAGRPAEMPEWIFGLWASGNEWNTQSLVMEQMDRHRNEGIPVSVVVIEAWSDEEGFTIFRDARYVPNQGQPHRGPDFTYPSDGAWPDPAGMIRELHERGIRVILWQIPL---QKTDDDL----GPEALAQGNALIASGHVVKEPDGTPYKNRGWWFPNALMPDLSTEAGRQWWTEQRRYLVEDLDIDG----------FKTDGGEHAWGSDLRYEDGRRGDEGNNLYPVNYARAYGDLLRSAGKYPVTFSRSGFTGSQAHGLYWA--------GDEDSTWEAFRSSITAGITAGACGI----LY---WGWDLAGFSGPVPEAELYARAFAAATF---MPIMQYHSEFHHHELPLRDRTPWNVAEQTGCGELIDLAR-HYTRVREALRPYLVAQTRQCLQTGKPLMRAMFYDHADDPEIWAHPRQYMLGDELLINPVTAPGATTWTTYLPEGQWEDYWSGEVSEGGHLVTRAVGWDIIPVYRRVG

General information: TITO was launched using: RESULT: Template: 5F7S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2564 -173057 -67.49 -326.52 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -67.49 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_5F7S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F7S-query.scw PDB file : Tito_Scwrl_5F7S.pdb: