Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYQEDLHPLLGKAVEHINRVMVGKRDIAILSLAALLAK-------GHVLLEDVPGVGKTMMVRALAKSIGADFKRIQFTPDLLPSDVTGVSIYNAKTMEFEYRPGPI---MGNIVLADEINRTSPKTQSALLEAMEEGSVTVDGHTMQLADPFFVMATQNPVEY-----EGTYPLPEAQLDRFLFKLRM-GYPSFNEELDVLSLQEKSHPIETLEPVIAKEDFIFLQRE----VQNVRADDSIKEYIVEIVQKTRQHPS-------VQLGVSPRGSIALMKAAQAYALLHHRDYVIPDDIQYLAPFTLPHRMMLHPEAKFEGIQAEAIVREIMSAVKVPVQRSAVR 3F8T Chain:A ((202-485)) -------KDPLTTFARAIAP-LPGAEEVGKMLALQLFSCVGKNSERLHVLLAGYPVVCSEILHHVLDHLAPRGVYV-DL-RRTELTDLTAVLKED---RGWALRAGAAVLADGGILAVDHLEGAPEPHRWALMEAMDKGTVTVDGI--ALNARCAVLAAINPGE-PSDPPIARIDLDQDFLSHFDLIAFLG--------------------------VPSYTLLRRYLLYAIREHPAPELTEEARKRLEHWYETRREEVEERLGMGLPTLPVTRRQLESVERLAKAHARMRLSDDVEPEDVDIAAELVDW-------------------------------------

General information: TITO was launched using: RESULT: Template: 3F8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1216 -182668 -150.22 -757.96 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -150.22 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3F8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8T-query.scw PDB file : Tito_Scwrl_3F8T.pdb: