Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKTKLVTDRIRLRCME-DRDQATLFG-LLFNDPDVMTYYSGLKDKRQTREWVN-WNQRNEKGYGVSLWIAEDKRTGEF-LGQCGIVPQQIENQT----VMEIGYMFARRHWGNGYAQEA-ARACLDYGFNERQFGKMAALIDPGNKASIRVAEKIGMHYSKTIRKWNKPIAVYERKSYN 3JUW Chain:A ((8-174)) ---RQVLKTDRLVLEPQSMARFDQWF-AMERQRDEAGHRDLTEDQAWLRLCARQGMWDAY---ACG--FYYLLDPVSG-EMRGEAGFQFRRRGFGPGFDNHPEAAWAVASAHQGRGLAAEAMQ-ALLAHHDRSSGRQRVVALIARSNLPSLRLAERLGFRGYSDVAFDGAAHLLLERA---

General information: TITO was launched using: RESULT: Template: 3JUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 653 -65267 -99.95 -413.08 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -99.95 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_3JUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JUW-query.scw PDB file : Tito_Scwrl_3JUW.pdb: