Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MQQHPDQLKLEEEPYLKGTVNTVIYHNDTNLYTVLKVKVTETSEAIEDKAVSVT------------------------GYFPALQ---EEETYTF---YGKIVTHPKFGL--------------QFQAEHFKKEIPTTKEGIIQYLSSDLFE-GIGKKTAEEIVKKLGDSAINKIL--------------ADASVLYDVPRLSKKKADTLAGALQRHQGLEQIMISLNQFGFGPQLSMKIYQAYE-SETLE------------------------KIQENPYQL-----------------------------------------VKDVEGIGFGKADELGSRMGLSGNHPERVK------------------------------AAILYTL---------------------ETTCLSEGH-TYIETEQLIIDTQSLLNQSAREGQRI---------------------------------------------TEMDAANAIIALGENKD-------IVIEDGRCYFPSL----------------------------------FYAEQNVAKRVKHIA------SQTEYENQFPESEFLLALGELEERMDVQYAPSQKEAIQKALSSP-MLLLTGGPGTGKTTVIRGIVELYGELHGVSLDPSAYKKDEAFPIVLAAPT--------GRAAK-----------RMSESTGLP-AVTIHRLLGWNGAEGFTHTE-DQPIEGK----LLIIDEASMLDIWLA-NHLFKAIPDHIQIIIVGDEDQLPSVGPGQVLRDLLASQVIPTVRLTDIYRQAEG--SSIVELAHQ----MKNGLLPNNLTAPTKDRSFIRCGGSQIKEVVEKVVANALKKGYTAKDIQVLAPMYRGKAGINELNVMLQDILNPPKEKRRELKFGDVVYRTGDKIL---QLVNQPENNVFNGDIGEITSIFYAKENTEKEDMAVVSFDGNEMTFTKKDFNQ--FTHAYCCSIHKSQGSEFPIVVLPVVK----GYYRMLRR--NLLYTAITRAKKFLILCGEEEALEW--GVKNNDATVRQTSLKNRLSVQVEEMDAELEALQKELPFSVHDANIGMEGITPFDFMKEEQQ 5EAN Chain:A ((1-1059)) GGHMEPLDELDLLLLEAVPRVELLRKKADAL-FPETVLSRGVDNRYLVLAVETSQNERGAEEKRLHVTASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRRAVLSETFRGSDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDEILCEVEEYLPSFSKWAEDFMRKGPSSE--FPQMQLSLPSDGRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVTVGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHLDKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIPEAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVSRVCDGQYLHNFQRKNGPMPATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTVFRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLN----KPQRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSAC-GFSVLLTSYTHSAVDNILLKLAKFKVGFLRLGQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQPVCLGPLFF----SRRFVLVGDHQQLPPLVVNREARALGMSE--------SLFKRLERNESAVVQLTVQYRMNRKIMSLSNKLTYAGK----LECGS--------DRVANAVLALPNLKDARLSLQLY---ADYSD-SPWLAGVLEPDNP---------VCFLNTDKVPAPEQVENGGVSNVTEARLIVFLTSTFIKAGCSPSDIGVIAPYRQQLRIISDLLARSSVGMVEVNTVDKYQGRDKSLILVSFVRSNEDGTLGELLKDWRRLNVALTRAKHKLILLGSVSSLKRFPPLGTLFDHLNAEQLILDLP------SREHESLSHIL-------------------------

General information: TITO was launched using: RESULT: Template: 5EAN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3306 106009 32.07 146.83 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 32.07 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_5EAN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EAN-query.scw PDB file : Tito_Scwrl_5EAN.pdb: