Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKYEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDP-EIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKP-YPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFSDFCPP 3LFS Chain:A ((1-292)) MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIR--------PSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTR-RALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKW-ARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKP

General information: TITO was launched using: RESULT: Template: 3LFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -165260 for 2167 contacts (-76.3/contact) + 2D Compatibility (PS) -30002 + (NN) -14957 + (LL) -316 1D Compatibility (HY) -34800 + (ID) 8550 Total energy: -253885.0 ( -117.16 by residue) QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_3LFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LFS-query.scw PDB file : Tito_Scwrl_3LFS.pdb: