Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAIAAITATSILALSACSSGDKEVIAKTDAGDVTKGELYTNMKKT------------AGASVLTQLVQEKVLDK-----KYKVSDKEIDNKLKEYKTQLGDQYTALEKQYGKDYLKEQVKYELLTQKAAKDNIKVTDADIKEYWEGL------KGKIRASHILVADKKTAEEVEKKLKK------GEKFEDLAKEYSTDS-SASKGGDLGWF-AKEGQMDETFSKAAFKLKTGEVS-DPVKTQYGYHIIKKTEERGK----YDDMKKELKSEVLEQKLNDNAAVQEAVQKVMKKADIEVKDKDLKDTFNTSSTSNSTSSSSSNSK 3RFW Chain:A ((22-273)) -------------------------ATVATVNGKSISDTEVSEFFAPMLRGQDFKTLPDNQKKALIQQYIMQDLILQDAKKQNLEKDPL-YTKEL--------------------DRAKDAILVNVYQEKIL-NTIKIDAAKVKAFYDQNKDKYVKPARVQAKHILVATEKEAKDIINELKGLKGKELDAKFSELAKEKSIDPGSKNQGGELGWFDQ--STMVKPFTDAAFALKNGTITTTPVKTNFGYHVILKENSQAKGQIKFDEVKQGIENGLKFEEF-KK-VINQKGQDLLNSAKVEYK-------------------------

General information: TITO was launched using: RESULT: Template: 3RFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 617 -45181 -73.23 -209.17 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -73.23 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.605

(partial model without unconserved sides chains): PDB file : Tito_3RFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RFW-query.scw PDB file : Tito_Scwrl_3RFW.pdb: