Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKEKAIVVFSGGQDSTTCLLWALKEFEEVETVTFHYNQRHSQEVEVAKSIAEKLGVKNHLLDMSLLNQLAPNALTRNDIEIEVKDGELPSTFVPGRNLVFLSFASILAYQIGARHIITGVCETDFSGYPDCRDEFVKSCNVTVNLAMEKPFVIHTPLMWLNKAETWKLADELGALDFVKNNTLTCYNGIIADGCGECPACHLRSKGYEEYMVMKGERA 2GOY Chain:A ((54-236)) --GDELWISFSGAEDV-VLVDMAWKLNRNVKVFSLDTGRLHPETYRFIDQVREHYGIAIDVLSPDP--RLLEPLVKEKG--LFSFYRDGHGECC---GIRKIEPLKRKLA--GVRAWATGQRRDQSPGTRSQVAV-LE-IDGAFS-TPEKPLYKFNPLSSMTSEEVWGYIRMLELPYNS---LHER--GYISIGCEPCTRPVL----------------

General information: TITO was launched using: RESULT: Template: 2GOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 794 -77408 -97.49 -422.99 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -97.49 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2GOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GOY-query.scw PDB file : Tito_Scwrl_2GOY.pdb: