Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLHISLEKKKVVIAGGGSIALRRLKTVISEGADITLVSPDVEPEIKQMAEERRIKWEKRTIE------KEDYLNAFFIIAATDNAAVNKEIAQSASP-F---QLVNCVSDAELGNVYMPKIVKRGHVTVSVSTSGASPKHTKELAENVDKLIDGDFVAEVNRLYQMRRKK 2G1U Chain:A ((16-149)) ------KQKSKYIVIFGCGRLGSLIANLASSSGHSVVVVDKNEY-AFHRLNSEFSGFTVVGDAAEFETLKECGMEKADMVFAFTNDDSTNFFISMNARYMFNVENVIARVYDPEKIKIFE-----ENGIK-TICP---AVLMIEKVKEFI----------------------

General information: TITO was launched using: RESULT: Template: 2G1U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 578 -72781 -125.92 -586.94 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -125.92 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_2G1U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G1U-query.scw PDB file : Tito_Scwrl_2G1U.pdb: