Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGMVLKPQPVFDAVEDLTSKAAAAPRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDERIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVLGKEASHIEDSFSTGLLEHPHYTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEWEKE 4IG6 Chain:A ((22-243)) MIFNVLTIFPQMFPGPLGVSNLGSALKKGLWTLNVFDIRAFANNKHNTVDDTPYGGGPGMLLRADVLGRCIDEVLSLHPNT-KLMFTSPRGVSFTQDIARQTMNFDNITLLCGRFEGIDERVVDFYKLQEVSIGDYVLSGGELAAMVIIDTCVRMVPGVIGNAESLKQESME-GSLEYPQYTRPASWKGMEVPEVLLTGNHGEIEKWRRNASLSITAARRPDLL-------------------

General information: TITO was launched using: RESULT: Template: 4IG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 918 -149074 -162.39 -671.50 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -162.39 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_4IG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IG6-query.scw PDB file : Tito_Scwrl_4IG6.pdb: