Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIKKFTAASMQEAALLIRKELGNEAVILNSKKIKKRKWFGLVNKPAVEVIAVLDQDFLEKKTPQKAAEPKQTLKTPVSSPKIEERTYPPQIPAQQELGDFSAYQSVLPEPLRKAEKLLQETGIKESTKTNTLKKLLRFSVEAG---GLTEENVVGKLQEILCDMLPSADK------WQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQLKTYAELLQAPLEVCYTKE----EFQQAKELF--SEYDHVFVDTAGRNFKDPQYIDELKETIPFESSIQSFLVLSATAKYEDMKHIVKRFS-SVPVNQYIFTKIDETTSLGSVFNILAESKIGVGFMTNGQNVPEDIQTVSPLGFVRMLCR 3E70 Chain:C ((52-324)) -----------------------------------------------------------------------------------------------------------VDKALDELEIDLLEADVALEVVDALREKIKQKLVGKKVRIG--GKIIEEAVKEAVSEILETSRRIDLIEEIRKAEKPYVIMFVGFNGSGKTTTIAKLANWLK-NHGFSVVIAASDTFRAGAIEQLEEHAKRIGVKVIKHSYGADPAAVAYDAIQHAKARGIDVVLIDTAGRSETNRNLMDEMKKIARVTKPNLVIFVGDALAGN-AIVEQARQFNEAVKIDGIILTKLDADARGGAALSISYVIDAPILFVGVGQGY-DDLRPFEKEWFLERIFG

General information: TITO was launched using: RESULT: Template: 3E70.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1297 -157583 -121.50 -620.40 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -121.50 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_3E70.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E70-query.scw PDB file : Tito_Scwrl_3E70.pdb: