Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK 2XY3 Chain:F ((1-128)) --MQIKIKYLDETQTRINK-EQGDWIDLRAAEDVAIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTKIKKGDRICQFRIMKKMPAVDLIEVDRL--------------

General information: TITO was launched using: RESULT: Template: 2XY3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 542 -63075 -116.37 -496.65 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain F : 0.94 3D Compatibility (PKB) : -116.37 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_2XY3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XY3-query.scw PDB file : Tito_Scwrl_2XY3.pdb: