Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLADKVKLSAK-EILEKEFKTGVRGYKQEDVDKFLDMIIKD---------YETFHQEIEELQQE--NLQLKKQLEEASKKQPVQSNTTNFDILKRLSNLEKHVFGSKLYD 2LSE Chain:A ((1-101)) MQEERKKLLEKLEKILDEVTDGAPDEARERIEKLAKDVKDELEEGDAKNMIEKFRDEMEQMYKDAPNAVMEQLLEEIEKLLKKAGS-----LVPRGSYLEHHHHHH----

General information: TITO was launched using: RESULT: Template: 2LSE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 277 41745 150.70 469.04 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 150.70 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_2LSE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LSE-query.scw PDB file : Tito_Scwrl_2LSE.pdb: