Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKVEKLSKSFGKHEVLKNISTTIAEGEVVAVIGPSGSGKSTFLRCLNLLEKPNGGTITIKDTEITKPKTNTLKVRENIGMVFQH--FHLFPHKTVLENIMYAPVNVKKESKQAAQEKAEDLLRKVGLFEKRND-YPNRLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVKEVLQVMKELVE-TGMTMVIVTHEMGFAKEVADRVLFMDQGMIVEDGNPKEFFMSPKSKRAQDFLEKIL 4MKI Chain:B ((27-245)) ----------------LKDINFSIDDEEFVGIIGHTGSGKSTLIQQLNGLLKPSKGKIYINGIDITDKKVSLKDIRKQVGLVFQYPEYQLF-EETVFKDIAFGPSNLGL-SEEEVKERVYEAMEIVGISKELADKSPFELSGGQKRRVAIAGILAMRPKILILDEPTAGLDPKGKQEILNKIKEIHDKYKMITILVSHNMEDIARIADKIIVMNRGKIELIGTPREVF------REAERLEKI-

General information: TITO was launched using: RESULT: Template: 4MKI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1005 -21459 -21.35 -99.81 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -21.35 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_4MKI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MKI-query.scw PDB file : Tito_Scwrl_4MKI.pdb: