Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLEKLRNLFGQLGIDGMLITSNTNVRYMTGFTGSA----GLAVIS--GDKAAFITDFRYTE-QAKVQVK--GFEIIEHGGSLIQT-TADTVESFGIKRLGFEQNSMTYGTYASYSAVISDAELVPVAESVEKLRLIKSSEEIKILEEAAKIADDAFRHILTFMKPGISEIAVANELEFYMRSQGADSSSFDMIVASGLRSSLPHGVASDKLIESGDLVTLDFGAYYKGYCSDITRTVAVGQPSDQLKEIYQVVFDAQALGVAHIKPGMTGKEADALTRDHIAAKGYGD-----YFGHSTGHGLGMEVHESPGLSVRSSAILEPGMVVTVEPGIYIPETGGVRIEDDIVITENGNRTITHSPKELIIL 4R60 Chain:A ((35-397)) QRIERARVLMRAQGVDALLIGAGTSLRYFSGVPWGASERLVALLLTTEGDPVLIC--PAFEEGSLDAVLQLPVRKRLWEEHEDPYALVVQAMDEQHAHALALDP-GIAFAVHTGLRAHLG-TAIRDAGAIIDGCRMCKSPAELALMQQACDMTLLVQRLAAGIAHEGIGTDQLVRFIDEAHRALGADNGSTFCIVQFGHATAFPHGIPGVQHLRAGELVLIDTGCTVQGYHSDITRTWIYGTPSDAQQRIWELELAAQAAAFAAVRPGVACEAVDQAARAVLQAAGLGPDYRLPGLPHRTGHGCGLAIHEAPYLVRGNRQPLQPGMCASNEPMIVVPGAFGVRLEDHFYVTDTGAQWFTPPSVAIDQ-

General information: TITO was launched using: RESULT: Template: 4R60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1908 -16708 -8.76 -48.01 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -8.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4R60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R60-query.scw PDB file : Tito_Scwrl_4R60.pdb: