Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKAEALAHTVIYRKNSRFDKVKEKSEELTLIGKGRSAYVFALTE--GGRKMALKVFFPEY---QATAVKEAAIYEKLAGSAFYPDIYETGD----SFILMEYIKGETFYNCLKKG-IAISD----DMIQQVEEALSDARAAGLNPSDIHLRNLILTET--GAVRVIDVARFEQTKTCTQWDDLKSAYHALYKKPI-----FPKKIPGFWLEIIAFLYKKDWFQKHFAQRKRKYS 1KOB Chain:A ((48-251)) ---------------------SVYDYYDILEELGSGAFGVVHRCVEKATGRVFVAKFINTPYPLDKYTVKNEISIMNQL-HHPKLINLHDAFEDKYEMVLILEFLSGGELFDRIAAEDYKMSEAEVINYMRQACEGLKHMHEHSIVHLDIKPENIMCETKKASSVKIIDFGLATKLNPDEI-VKVTTATAEFAAPEIVDREPVGFYTDMWAIGVLGYVLLSGLSPFA---------

General information: TITO was launched using: RESULT: Template: 1KOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 714 -16908 -23.68 -92.39 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -23.68 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_1KOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KOB-query.scw PDB file : Tito_Scwrl_1KOB.pdb: