Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIKIDDLTGRQVVSLVNEHLHSMTLMSPPESIHALGLEKLRGPEIT----FWSAWEGDELAGCGALKEL-DTRHGEIKSMRTSASHLRKGVAKQVLQHIIEEAEKRGYERLSLETGSMASFEPARKLYESFGFQYCEPFADYG--EDPNSVFMTKKL- 2FIA Chain:A ((1-157)) MKIRVADEKELPMILQFLTEVKAYMDVVGITQWTKDYPSQGDIQEDITKKRLYLLVHEEMIFSMATFCMEQEQDFVWLKRFATSPNYIAKGYGSLLFHELEKRAVWEGRRKMYAQTNH--TNHRMIRFFESKGFTKIHESLQMNRLDFGSFYLYVKELE

General information: TITO was launched using: RESULT: Template: 2FIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 599 -1121 -1.87 -7.52 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -1.87 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_2FIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FIA-query.scw PDB file : Tito_Scwrl_2FIA.pdb: