Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEQPIGVIDSGVGGLTVAKEIMRQLPKENIIYVGDTKRCPYGPRPEEEVLQYTWELTNYLLENHHIKMLVIACNTATAIALDDIQRSVGIPVVGVIQPGARAAIKVTDNQHIGVIGTENTIKSNAYEEALLALNPDLKVENLACPLLVPFVESGKFLDK--TADEIVKTSLYPLKDTSIDSLILGCTHYPILKEAIQRYMGEHVNIISSGDETAREVSTILSYKGLLNQSPIAPDHQFLTTGARDQFAKIADDWFGHEVGHVECISLQEPIKR 1B74 Chain:A ((106-213)) -------------------------------------------------------------------------------------------------------------NKKIGVIGTPATVKSGAYQRKLEEG--GADVFAKACPLFAPLAEEGLLE--GEITRKVVEHYLKEFK-GKIDTLILGCTHYPL-LKKEIKKFLGDAEVVDSSEALSLSLHNFIK---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1B74.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 373 -10443 -28.00 -98.52 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -28.00 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_1B74.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B74-query.scw PDB file : Tito_Scwrl_1B74.pdb: