Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLVLIGGGYGNMRVLHRLLPNQLPDDVSITLIDRNPYHCLKTE-Y-YALAAGTISDHHIRVSFPEHP---RLDVQYGDITSIDIVQKQVLFQDREPISYDDAIIGLGCEDKYHNVPGAPE-FTYSIQTIDQSRETYQKLNNLSANATVAIVGAGLSGVELASELRESRDDLNIILFDRGNLILSS-FPERLSKYVQKWFEEHGVRIINRANITKVEEG------VVYNHDDPISADAIVWTAGIQPN-KVVRDLDVEKDAQGRIVLTPHHNLPGDEHLYVVGDCASLPH--------APSAQLAEAQAEQIVQILQKRWNGEALPESMPQFKLKGVLGSLGKKAGFGLVADRPLIGRVPRMLKSGLLWMYKHHNG 3FG2 Chain:P ((1-310)) NDTVLIAGAGHAGFQVAVSLRQAK--YPGRIALINDEKHLPYQRPPLSKAYLKSGGDPNSLMFRPEKFFQDQAIELISDRMVSIDREGRKLLLASGTAIEYGHLVLATGARNRMLDVPNASLPDVLYLRTLDESEVLRQRM---PDKKHVVVIGAGFIGLEFAATARAKG--LEVDVVELAPRVMARVVTPEISSYFHDRHSGAGIRMHYGVRATEIAAEGDRVTGVVLSDGNTLPCDLVVVGVGVIPNVEIAAAAGLPTA--AGIIVDQQLL-TSDPHISAIGDCALFESVRFGETMRVESVQNATDQARCVAARLTGD---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1527 -5702 -3.73 -19.80 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain P : 0.68 3D Compatibility (PKB) : -3.73 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3FG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FG2-query.scw PDB file : Tito_Scwrl_3FG2.pdb: