Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGNNAGHTIKF-DGITYKLHLIPSGIFYKDKTCVIGNGMVVDPKALVTELAYLHERNVSTDN-LRISNRAHVILPYHLKLDEVEEERKGANKIGTTKKGIGPAYMDKAARIGIRIADLLDRDAFAEKLERNLEEKNRLLEKMYETEG-FKLEDILDEYYEYGQQIKKYVCDTSVVLNDALDEGRRVLFEGAQGVMLDIDQGTYPFVTSSNPVAAVSQSVLVSARPKIKHVVGVSKAYTTRVGDGPFPTELKDEIGDQIREVGREYGTTTGRPPRVGWFDSVVVRHARRVSGITDLSLNSIDVLAGIETLKICVRYRYK-GEIIEEFPASLKALAECEPVYEEMPGWTEDITGAKSLSELPENARHYLERVSQLTGIPLSIFSVGPDRSQTNVLRSVYRAN 1QF5 Chain:A ((1-431)) GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVIN-GEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAV-DYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGAGPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLDVLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGLPQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA-

General information: TITO was launched using: RESULT: Template: 1QF5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2537 12767 5.03 29.90 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 5.03 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_1QF5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QF5-query.scw PDB file : Tito_Scwrl_1QF5.pdb: