Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKRITFGLLVLMVCVILFVLYVQLR--NKDSIPVQSSAI-----------------HPEED--RIFFIYSNPFIKESVLLSTSTGERFNRRTFK-----------------VADVPYIQTKSYAST----DLVLLAEHEPF-------------YYTLEKDVIKEHPLSDPFAFWYEGKDVTIEAYNVDTTGNEIHI------------NDRKTKKEYTLTLPPLVTMGAS------------------------------DENF-------IYIIQSMSIYVIDRKTEEMIETLSLASYADQFADSEEFIVASSDHKLTVIEKGTWKTT--YIAYPEDLEYADTVYYDKESGSFYVAYEDKEGG--------ANLLEYGEDFSI-HTYSLKFPYMEAKFKGN--------LLYIVAQEEHKK-------GIGGYVGVFDIHSKEMLYQFDLPEEQVKVQDFVVVDNK 2Q06 Chain:A ((22-496)) ATEIRASVGRMVGGIGRFYIQMCTELKLSDQEGRLIQNSITIERMVLSAFDERRNRYKDPKKTGGPIYRRRDGKWVRELILYDKEEIRRIWRQANNGEDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAIKGVGTMVMELIRMIKRGINDRNFWRGENGRRTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEIEDLIFLARSALILRGSIAHKSCLPACVYGLAVASGYDFEREGYSLVGIDPFRLLQNSQVFSLIRPNENPAHKSQLVWMACHSAAFEDLRVSSFIRGTRVIPRGQLSTRGVQIASNENVEAMDSSTL--ELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFERATIMAAFKGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEY-

General information: TITO was launched using: RESULT: Template: 2Q06.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1171 -50497 -43.12 -149.84 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -43.12 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.045

(partial model without unconserved sides chains): PDB file : Tito_2Q06.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q06-query.scw PDB file : Tito_Scwrl_2Q06.pdb: