Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRP--QGIPVHEDFRFETIPVPEP-KQGEVLVKTLYVSVDPYMRGRM-QDTKS-YVEPFALDKALSGGVIAEVVSDGNH--LKKGDIVIG-NLSWQEFSAVSESALRKIDTSLAPA--SAYLGILGMTGLTAYFGLLDIGRPKEG--ETVVVSGAAGAVGSTVGQIAKIKG-ARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAE-SEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 2W98 Chain:B ((5-348)) -----QRVVLNSRPGKNGNPVAENFRMEEVYLPDNINEGQVQVRTLYLSVDPYMRCRMNEDTGTDYITPWQLSQVVDGGGIG-IIEESKHTNLTKGDFVTSFYWPWQTKVILDGNSLEKVDPQLVDGHLSYFLGAIGMPGLTSLIGIQEKGHITAGSNKTMVVSGAAGACGSVAGQIGHFLGCSRVVGICGTHEKCILLTSELGFDAAINYK-KDNVAEQLRESCPAGVDVYFDNVGGNISDTVISQMNENSHIILCGQISQYNKDVPYPPPLSPAIEAIQKERNITRERFLVLNYKDKFEPGILQLSQWFKEGKLKIKETVINGLENMGAAFQSMMTGGNIGKQIVCISE--

General information: TITO was launched using: RESULT: Template: 2W98.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1966 -196169 -99.78 -594.45 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -99.78 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_2W98.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W98-query.scw PDB file : Tito_Scwrl_2W98.pdb: