Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVMLATALFLGLTPAGANAADLGHQTLGSNDGWGAYS-----TGTTGGSKASSSNVYTVSNRNQLVSALGKETNTTPKIIYIKGTIDMNVDDNLKPLGLNDYKDPEYDLDKYLKAYDPSTWGKKEPSGTQEEARARSQKNQKARVMVDIPANTTIVGSGTNAKVVGGNFQIK-SDNVIIRNIEFQDAYDYFPQWDPTDGSSGNWNSQYDNITINGGTHIWIDHCTFNDGSRPDSTSPKYYGRKYQHHDGQTDASNGANYITMSYNYYHDHDKSSIFGSSDSKTS--DDGKLKITLHHNRYKNIVQRAPRVRFGQVHVYNNYYEGSTSSSS---YPFSYAWGIGKSSKIYAQNNVIDVPGLSA-AK--T--I-SVFS-GGTALYDS----GTLLNGTQINASAANGLSSSVGWT--PSLHGSIDASANVKSNVINQAGAGKLN 3ZSC Chain:A ((4-332)) -----------------------------DKPVGFASVPTADLPEGTVGGLGGE---IVFVRTAEELEKYTTAE---GKYVIVVDGTIVFEPK-----------------------------------------------------REIKVLSDKTIVGIN-DAKIVGGGLVIKDAQNVIIRNIHFEGFYMED---DP-----RGKKYDFDYINVENSHHIWIDHITFVNG-----------------NDGAVDIKKYSNYITVSWNKFVDHDKVSLVGSSDKEDPEQAGQAYKVTYHHNYFKNLIQRMPRIRFGMAHVFNNFYSMGLRTGVSGNVFPIYGVASAMGAKVHVEGNYFMGYGAVMAEAGIAFLPTRIMGPVEGYLTLGEGDAKNEFYYCKEPEV-RPVEEGKPALDPREYYDYTLDPVQDVPKIVVDGAGAGKLV

General information: TITO was launched using: RESULT: Template: 3ZSC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2078 -120117 -57.80 -393.83 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -57.80 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3ZSC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZSC-query.scw PDB file : Tito_Scwrl_3ZSC.pdb: